First records of Phytophthora spp. based on DNA analysis in Lithuania
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vitas-12-1-3.pdf (1.76 MB)
Date
2012
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Instytut Badawczy Leśnictwa, Komitet Nauk Leśnych PAN
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Abstract
The assessment of alien invasive species of Phytophthora genus causing serious forest tree species diseases was carried out in Lithuania. The presence of Phytophthora DNA was recorded for the first time using real-time PCR analysis on 23 DNA samples. The sampling includes wood from diseased trees, leaves from shrubs, leaves baited in water, and soil samples taken around diseased plants. Extracted DNA from soil and plant tissues was tested for the presence of Phytophthora. All analysed samples were positively recognized by Phytophthora-specific probe during real-time PCR which proves the presence of pathogens in environmental samples.